ORCHID MEDIA Formulation

The standard media reference is given along for documentation purposes.

MEDIA FORMULATIONS GUIDE FOR ORCHIDS
	KNUDSON MODIFIED MEDIA- K4003
Sno	Component	Code		mg /L
1	Ammonium Sulfate			500
2	Boric acid			0.056
3	Calcium Nitrate 4H20			694.4
4	Cupric Sulfate.5H2O			0.0624
5	Ferrous sulfate.7H2O			25
6	Magnesium Sulfate			122.125
7	Manganese Sulfate.H2O			5.682
8	Molybdenum TriOxide			0.016
9	Potassium Phosphate MonoBasic			250
10	Zinc Sulfate.7H2O			0.331
	ORGANICS
	Sucrose			20,000
	KNUDSON MOREL MODIFIED MEDIA- K4128
Sno	Component	Code		mg /L
1	Ammonium Nitrate			500
2	Ammonium Sulfate			500
3	Calcium Nitrate 4H20			347.2
4	Ferrous sulfate.7H2O			25
5	Magnesium Sulfate			122.125
6	Manganese Sulfate.H2O			5.682
7	Potassium Phosphate MonoBasic			250
8	Potassium Chloride			250
	ORGANICS
	Sucrose			20,000
	Lindemann Orchid Basal Salt Mixture-L0270
Sno	Component	Code		mg /L
1	Aluminum Chloride 6H2O			0.05611
2	Ammonium Sulfate			1000
3	Boric Acid			1.014
4	Calcium Nitrate 4H20			347.2
5	Cupric Sulphate 5H2O			0.019
6	Ferric Citrate			4.4
7	Magnesium Sulfate			58.62
8	Manganese Sulfate.H2O			0.05153
9	Nickel Chloride. 6H2O			0.03116
10	Potassium Chloride			1050
11	Potassium Iodide			0.099
12	Potassium PhosphateMonoBasic			135
13	Zinc Sulphate.7H2O			0.565
	ORGANICS

Ready Media is easily available from HIMEDIA labs for ordering. Please email us for more details.

We use a product that is to prevent contamination of cultures:

Applicants have surprisingly discovered that these combinations of chemicals, in a particular range of concentrations, are effective in reducing or preventing microbial contamination in plant tissue cultures for the duration of the tissue culture period, but allow for substantially normal germination of seeds or substantially normal growth or development of plants, plant organs, plant tissues or plant cells.

The relative concentrations of the individual components comprising the chemical agent may be varied to produce a mixture that is optimally effective in practicing the method of the present invention for any particular plant culture medium, plant species, plant seed, plant, plant organ, plant tissue or plant cell.

However, a preferred mixture of the components in the chemical agent comprises: methylchloroisothiazolinone in a concentration range of about 2.0 to about 2.6 g/l; methylisothiazolinone in a concentration range of about 0.6 to about 0.8 g/l; magnesium chloride in a concentration range of about 15.0 to about 30 g/l; and magnesium nitrate in a concentration range of about 15.0 to about 30 g/l. A more preferred mixture of the components in the chemical agent further comprises: potassium sorbate in a concentration range of about 15 to about 25 g/l or sodium benzoate in a concentration range of about 13 to about 27 g/l. A most preferred mixture of the components in the chemical agent further comprises: potassium sorbate in a concentration range of about 15 to about 25 g/l; and sodium benzoate in a concentration range of about 13 to about 27 g/l.

In all cases, the components of the chemical agent are mixed to form a stock solution of chemical agent using any liquid in which the components will dissolve, but preferably in water, and most preferably in distilled or deionized water. As used in the present application, "plant tissue culture" or "culturing plant tissues" refers to any process carried out in vitro wherein seeds are germinated, or plants, plant organs, plant tissues or plant cells are propagated, differentiated, subcultured or otherwise maintained on a culture medium, defined or undefined, which typically is maintained in a sterile (syn: aseptic, axenic) condition, i.e., free of microbial contamination, and generally is incubated under controlled environmental conditions.

As used in the present application, "microbial contamination" refers to the growth of any unwanted microorganisms, e.g., bacteria or fungi, in a plant tissue culture.

As used in the present application, the terms "plant tissue culture medium," "plant culture medium," "culture medium," and "medium" refer to a solid substrate or liquid solution in which a plant seed will germinate, a plant can be maintained or grown, an isolated plant organ or plant tissue can be maintained, propagated or differentiated, or one or more isolated plant cells, plant cell aggregates or plant cell protoplasts may be maintained, propagated, or differentiated, and which is to be maintained in a sterile condition, i.e., substantially free of microbial contamination.

Generally, the chemical agent useful in the method of the present system will be effective in a range of concentrations in the culture medium that allow for substantially normal seed germination or substantially normal growth or development of a plant, plant organ, plant tissue or plant cell cultured thereon. A preferred range of concentrations of the chemical agent in the culture medium is from about 0.01% to about 0.20% (v/v). A more preferred range of concentrations of the chemical agent in the culture medium is from about 0.02% to about 0.10% (v/v). The most preferred range of concentrations of the chemical agent in the culture medium is from about 0.03% to about 0.05% (v/v).

In a normal set of usage, chemical agent comprising the tissue protection chemical was prepared for incorporation into the culture medium prior to autoclaving.

Incase you have requirements for these mixtures please email us.

SETTING UP A TC LAB FOR ORCHIDS AND FOLIAGE

MEDIA FORMULATIONS

MERICLONING

ORCHID TC CHEMICALS

ORCHID SEED PROPAGATION

ORCHID KEIKEIS

VEGETATIVE MULTIPLICATION

Flasking-PRICE LIST